Abstract: Eco-industrial chain is an important part in the building of the eco-Industrial park.On the basis of the MFA which is combined with the Auto Eco-Industrial Chains of TEDA of Tian Jin,this paper makes coupling analysis of the industrial chain by the PIOT,and builds the model of MFA of the industrial chain which is based on the three-dimension PIOT.The model specifies and quantifies the process of the material metabolizing,characteristic of the structure,consumption of the resource,and discharge of the waste in the industrial chain and offers technical support to the building of the eco-industrial chains and the introduction of the complementary enterprises.