Abstract: Online teaching is highly valued because of the impact of the COVID-19 epidemic, which has hampered normal teaching activities in colleges and universities. At the same time, the virtual simulation experimental teaching has become an important part of online experimental teaching in universities for the students can feel the realistic experimental scenes to carry out autonomous experiments in the virtual experimental environment. According to the analysis of the status of virtual simulation experimental teaching resources’ development at N University, there are still some problems in the development of virtual simulation experimental teaching resources in universities: small numbers of resources, an uneven distribution of resources, low sharing of resources and low utilization of resources. Thus a series of targeted strategies are proposed, which can provide some recommendations for the sustainable development of virtual simulation experimental teaching in universities.