Abstract: To improve adoption level and application effect of RFID, the STOPE framework was used to analyze the influencing factors of RFID adoption theoretically under the four adoption levels of unit level, tactical level, strategic level and manufacturing network level, and the research hypotheses were put forward. On this basis, the STOPE-AM model was constructed, and the structural equation model was used to test the theoretical model according to the data of 357 questionnaires. The results show that the key influencing factors of manufacturing enterprises’ RFID adoption under different adoption levels are different, the higher the adoption level, the more complex the key influencing factors.