The Performance of UVB Resistance of Lactobacillus paracasei VHProbi E12 Fermented Agastaggosa rugosa Extract

Ultraviolet B (UVB) irradiation can make cell generate free radicals, thereby leading to cellular senescence and photoaging. In recent years, due to environmental pollution and the destruction of the ozone layer, the intensity of UVB radiation has increased, exacerbating the problem of skin photoaging. To screen for fermented Agastache rugosa strains with anti-photoaging effects, a UVB damage model of human immortalized keratinocytes (HaCaT) was established. The efficacy of fermentation is evaluated based on cell viability, anti-photoaging, antioxidant and anti-inflammatory effects. The results indicate that Lactobacillus paracasei VHProbi E12 fermented Agastache rugosa extract helps HaCaT resist UVB damage and increases cell viability by 21.20%. In terms of anti-photoaging effect, high-dose E12 fermented Agastache rugosa extract reduces the matrix metalloproteinase-1content in HaCaT by 24.68%, and increases the hydroxyproline concentration by 43.79% compared with the model group. Regarding antioxidant capacity, E12 fermentation treatment can reverse the oxidative damage caused by UVB irradiation by elevating the levels of SOD acitivity and GSH content in cells. Compared with the model group, the high-dose E12 fermented Agastache rugosa extract increases the SOD by 77.75% and GSH content by 26.03%. Additionally, after E12 fermentation broth treatment, the inflammation caused by UVB irradiation is also alleviated, evidenced by reduced levels of TNF-α and IL-6 compared with the untreated group. This study provides evidence that a Agastache rugosa extract fermented by Lactobacillus paracasei VHProbi E12 protects HaCaT cells against UVB damage, and provides a theoretical basis for the further development of Agastache rugosa and the utilization of anti-photoaging natural extracts in sunscreen products.