Screening and Reuse of Formaldehyde-Degrading Bacteria in Rhizosphere Microorganisms of Epipremnum Aureum

Combinationusing plants with microorganisms to gaseoustreat formaldehyde was considered to be an efficient, economical and environmentally friendly method.In this paper, a formaldehyde-degrading bacterium LL3-1(gram-positive and facultative aerobic) was successfully isolated from the rhizosphere microbial community of Epipremnum Aureum. From the Scanning electron microscope (SEM) photos, the bacterium LL3-1 were rod-shaped, opaque, with diameter ranging from 712.8 nm to 1.146 μm, and width ranging from 199.4 nm to 495.5 nm. Byusing 16S rDNA technology, the bacterium LL3-1was identifiedas a Lysinibacillus endophyticus strain (with a unique GenBank accession number of MN784374). From results of the liquid formaldehyde degradation experiment, LL3-1 strain can completely degrade formaldehyde solution up to 500 mg/L in 6 hours under the environment of 35 ℃, a rotational speed of 150 r/minand a pH7.0. Finally, the 100 mL bacterial liquidwithin culture mediumwas sprayed on the leaves of Epipremnum Aureum, and the dynamic fumigation system was used to explore the potential of LL3-1 strain combined with Epipremnum Aureum in purifying gaseous formaldehyde both in daytime and at night. The results showed that after two time of spraying both in the daytime (7:00) and at night (19:00), the average formaldehyde purification efficiency of Epipremnum Aureum increased by 33.1% ± 1.28% in daytime and 12.17% ± 2.25% at night. This indicates that strain LL3-1 can not only degrade high concentration formaldehyde in solution, but also has great potential in treating air pollutants in combination with plants.