WANG Xiao-ming, CHEN Su-hua, WU Guo-ping, Su Xiang-xu, BI Qian-qian, WU Qing-qing. Lsolation and Characterization of Pepsin-solubilized Collagen From the Skin of the Black Carp[J]. Journal of nanchang hangkong university(Natural science edition), 2014, 28(1): 53-59. DOI: 10.3969/j.issn.1001-4926.2014.01.010
Citation: WANG Xiao-ming, CHEN Su-hua, WU Guo-ping, Su Xiang-xu, BI Qian-qian, WU Qing-qing. Lsolation and Characterization of Pepsin-solubilized Collagen From the Skin of the Black Carp[J]. Journal of nanchang hangkong university(Natural science edition), 2014, 28(1): 53-59. DOI: 10.3969/j.issn.1001-4926.2014.01.010
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Lsolation and Characterization of Pepsin-solubilized Collagen From the Skin of the Black Carp

    Abstract
  • Pepsin soluble collagen (PSC) was successfully isolated from the skin of black carp with a yield of 85% based on dry weight. The collagen contained two identical alpha 1-chains and an alpha 2-chain with no disulfide bond.The purified PSC was characterized as typeⅠand contained imino acid of 133 imino acid residues vs. 1000 residues. The denaturation temperature of this collagen was 25.6℃, which was lower than that of porcine collagen by approximately 11℃. The isoelectric point (pI) was estimated to be 8.23. The ultraviolet (UV) absorption spectrum of PSC showed that the distinct absorption was at 218nm. Maximum solubility of this collagen in 0.5 mol/l acetic acid was observed at pH 3. PSC lost the solubility when salt concentrations increased. The high yield showed that it is possible to use black carp skin collagen as an new source for industrial purposes.
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