Abstract: Separation and analysis of trace components in sophisticated matrices is the most severe challenge which separation science encounters.As a result,modern micro-separation technology has become the research hotspot in chemistry,chemical engineering and life science.In this paper,the new technologies on modern micro-separation and their development in the latest 5 years,including capillary electrophoresis,capillary electrochromatography,microfluidic chip and hyphenation of mass spectrometry,were introduced.The recent application of these new technologies in environmental and biomedicine analysis was reviewed.Its development prospect in these fields was briefly outlined (with 48 references).