Abstract: Reconstructing 3D motion and structure based on optical flow becomes the hot point and frontier in the research of computer vision.It is divided into linear and unlinear algorithms.The choice of initial value of the unlinear algorithm is important,otherwise algorithm fails.We present a new experiment simulation method to prove linear algorithm,which determines 3D motion and structure based on optical flow.The presented Experimental method using synthetic data and image sequences provided a good estimation of the optical flow and 3D motion and structure.