Abstract: In order to study the interaction between two spheres and the effect of different space on wake and turbulent kinetic energy,numerical simulations on five cases between L=2.0D and 4.0D were performed by Computational Fluid Dynamics at Rep≈1000.Numerical simulation showed that streamwise transport sphere is reciprocally restrained by two spheres.Spanwise transport of sphere is reciprocally augmented by two spheres.It was observed that the second vortex occurred at the distance ratio of L≥3.0D.The magnitude of turbulence enhancement decreases with the increase of sphere distance roughly in a square law way.