Abstract: With the development of science and technology, the application of L-lactic acid is wider and wider. And the treatment of residue from refining L-lactic acid is also noticed by people. The authors want to isolate a kind of effective bacteria in the biotreatment of residue from refining L-lactic acid, and then make sure its favorable environment through experiments. And authors also wish this experimental result can benefit to the future study and the practical application in engineering.