Abstract: In order to study the indoor thermal comfort state in hot summer and cold winter areas and save energy consumption, the reading room of a university in Nanchang was used as the research object. The indoor temperature, humidity and wind speed were measured for three consecutive days. The PMV index value of the room was calculated to obtain the predicted neutral temperature, which is 18.9 °C. Using ASHRAE seven-level thermal sensation index to conduct a questionnaire survey on the thermal comfort of the room, get 205 answers. Calculating the TSV, TCV, and fit the TSV straight line to get the neutral temperature of the reading room is 17.25 ℃, which is lower than the expected temperature, indicating that students are adapting to the colder environment. The neutral temperature provides a reference for the room air conditioning temperature setting, and tries to calculate the saved energy. It is measured that the concentration of carbon dioxide in the reading room is too high and the comfort level is low, so proper ventilation should be provided in this room.