Abstract: Aiming at high requirement of center line extraction of AGV in precision based on guided ribbon, a method of guided ribbon recognition and center line extraction is designed by using the principle of computer vision. For the first, the images collected by camera is conducted by a serial of proceedings including Graying, Median filter, image threshold segmentation and boundary of the segmented image detection. Then a method of guided ribbon’s center line extraction based on geometric construction is proposed, figuring out a serial of information such as deflection and offset distance and so on according to center line equation. Ultimately, mentioned processes are blended to design a system of guided ribbon’s center line extraction based on MATLAB.