Abstract: With the rapid upgrading aircraft types and development of assembly technology in China, the shortcomings of the obsolete, aging and teaching and small quantity of equipments have become increasingly prominent. Therefore, a new teaching method of Aircraft Assembly Technology course is proposed, which integrates the CDIO education concept and virtual simulation. Using virtual simulation teaching to increase the quantity and improve technical backwardness of experimental equipments for aircraft assembly teaching. The CDIO education concept is introduced to guide the virtual simulation teaching of Aircraft Assembly Technology course. A level 3 virtual simulation experiment practice project is constructed to transform students from passive learning to active thinking, and from passive acceptance to independent experimental verification.