Abstract: The extraction method of laser stripe center based on hessian matrix algorithm produces miscellaneous points because of the noise, the accuracy of laser stripe center will be reduced. Therefore, an extraction method of hessian matrix laser stripe centre based on difference image method was presented. The method of image morphology was used to remove the part of the noise. The difference image method was used to acquire the laser stripe target area. The Hessian matrix and the Taylor series were used to Gaussian convolution and Taylor expansion in the laser stripe target area, and the center of the laser stripes was extracted. The algorithm was evaluated from the aspects of processing speed and extraction precision. The results show that the accuracy of the proposed method is improved the 30%, and the speed is improved 5 times, compared with the hessian matrix method, and improved the speed and accuracy of extraction.