Abstract: 3D printing technology has such advantages as rapid prototyping, low cost and high integration, and can be applied to prepare complicated parts. It has advanced fast in the recent years, but still has some disadvantages. For example, its accuracy is not high enough, and the properties of materials by 3D printing are instable. With the extensive application of the two-phase titanium alloys by 3D printing, researches on the two-phase titanium alloys by 3D printing have been put more emphasis on both at home and abroad. In this paper, the principles of laser rapid prototyping and electron beam melting prototyping, two prototyping methods of 3D printing technology, were introduced. The microstructures and properties of the two-phase titanium alloys prepared by laser rapid prototyping and electron beam melting prototyping respectively were analyzed and compared. In addition, the effect of processing parameters and heat treatment on the microstructures and properties of the alloys was also discussed.