Abstract: According to the vibration fatigue problem of hypersonic aircraft under the thermal-mechanical environment, impacts of the temperature change on vibration properties and fatigue life of aircraft panel are studied. Firstly, the temperature field and stress field are obtained by analyzing three dimension transient coupling thermal conduct and thermal stress. Then, impacts of the temperature and stress and theirs coupling on panel's vibration mode and fatigue life are discussed. During the analysis, the stiffness of panel material influenced by the temperature is considered. Initial stress additional stiffness matrix caused by thermal stress and initial displacement stiffness matrix caused by thermal strain are introduced. Results indicate that different modes the panel are going to be decreased because the performance is worsen by the action of temperature. Impacts of temperature grade on vibration mode are obvious. The vibration fatigue life is shortened because of the coupling effect.